Simulating DNA Paternity Test

€158,60 incl. VAT

With this package students will conduct a simplified version of the DNA fingerprint analysis process and determine the likelihood that the alleged father is the biological father. Generally 3-6 matching DNA probes are required to produce a 99.0% first degree affinity.

We simulate how fingerprint DNA can be used to determine the genetic relationship between a child and a presumed father.

He will use the effect of a human DNA muscle electrophoresis, heredity and scientific skills to solve a hypothetical scenario.

Set for the whole class.

Before fingerprint DNA was discovered, the paternity test was based on blood type. This was because a person’s blood type is derived from his parents’ blood type. However, as many people have the same blood type, this test can be used to rule out potential biological parents but not to prove who they really are.

DNA analysis of fingerprints on the other hand can prove the degree of affinity with great certainty.

With this package, students will perform a simplified version of the DNA analysis process from fingerprints and determine the likelihood that the alleged father is the biological father. Generally 3-6 matching DNA probes are required to produce a 99.0% first degree affinity.

The kit includes:

150 μL of parent DNA

150 μL of (supposed) paternal DNA

150 μL of child DNA

125mL of 20X concentrated TBE buffer

400 mL agarose gel 0.8%

100 mL of 10X blue concentration DNA labeling solution

1 plastic tray

Instructions for the teacher (in English)

Student worksheets (in English)

Materials needed but not included:

Gloves

Protective glasses

Protection apron

Micropipettes 10μL

Tips for micropipette

Electrophoresis device

The package can support the whole class.

Laboratory preparation by the teacher is recommended.

Although DNA samples are stable at room temperature, it is recommended that they be refrigerated before use.
DNA samples contain sucrose dye. Sucrose makes the DNA sample denser than the buffer and allows it to sink to the bottom of the well. The dye allows the movement of DNA to be monitored.
The remaining reagent is stored at room temperature.

Laboratory Preparation

TBE buffer

Dilute 20mL of the 20X buffer with 380mL of deionized water. This resulting 400mL solution is sufficient to make two gels.

Agarozi gel

Ready-made agarose gels can be stored at room temperature. Prior to electrophoresis, place in a microwave oven for 1 minute until completely liquefied. Allow to cool to 55 degrees Celsius.

DNA labeling solution

Dissolve 10 mL of the concentrated label solution in 90 mL of hot tap water solution.

The DNA samples provided in the kit are not of human origin. They come from a bacteriophage and do not require any special treatment. The band pattern resulting from the electrophoresis is representative of the actual result.

https://www.youtube.com/watch?v=HdzBh6sMLA0